Enhanced cellulosic d-lactic acid production from sugarcane bagasse by pre-fermentation of water-soluble carbohydrates before acid pretreatment.

After several rounds of milling process for sugars extraction from sugarcane, certain amounts of water-soluble carbohydrates (WSC) still remain in sugarcane bagasse. It is a bottleneck to utilize WSC in sugarcane bagasse biorefinery, since these sugars are easily degraded into inhibitors during pretreatment. Herein, a simple pre-fermentation step before pretreatment was conducted, and 98 % of WSC in bagasse was fermented into d-lactic acid. The obtained d-lactic acid was stably preserved in bagasse and 5-hydroxymethylfurfural (HMF) generation was sharply reduced from 46.0 mg/g to 6.2 mg/g of dry bagasse, after dilute acid pretreatment. Consequently, a higher d-lactic acid titer (57.0 g/L vs 33.2 g/L) was achieved from the whole slurry of the undetoxified and pretreated sugarcane bagasse by one-pot simultaneous saccharification and co-fermentation (SSCF), with the overall yield of 0.58 g/g dry bagasse. This study gave an efficient strategy for enhancing lactic acid production using the lignocellulosic waste from sugar industry.

One-pot fermentation for erythritol production from distillers grains by the co-cultivation of Yarrowia lipolytica and Trichoderma reesei.

A co-fermentation process involving Yarrowia lipolytica and Trichoderma reesei was studied, using distillers grains (DGS) as feedstocks for erythritol production. DGS can be effectively hydrolyzed by cellulase in the single-strain culture of T. reesei. One-pot solid state fermentation for erythritol production was then established by co-cultivating Y. lipolytica M53-S with the 12 h delay inoculated T. reesei Rut C-30, in which efficient saccharification of DGS and improved production of erythritol were simultaneously achieved. The 10:1 inoculation proportion of Y. lipolytica and T. reesei contributed to the maximum erythritol production of 267.1 mg/gds under the optimal conditions including initial moisture of 55%, pH of 5.0, NaCl addition of 0.02 g/gds and DGS mass of 200 g in 144 h co-cultivation. Being compared with the attempts to produce erythritol from other raw materials, the one-pot SSF with DGS is proposed to be a potential strategy for efficient and economical erythritol production.

Enhanced efficiency of enzymatic hydrolysis of wheat straw via freeze-thaw pretreatment.

This research investigated enhancing the efficiency of enzymatic hydrolysis of wheat straw via freeze-thaw pretreatment and assessed the physicochemical structural changes after this pretreatment. The enzymatic hydrolysis efficiency of cellulose and hemicellulose was enhanced, and hemicellulose was more susceptible to pretreatment. The highest enzymatic hydrolysis efficiency of cellulose and hemicellulose was 57.06 and 70.66%, respectively, at - 80 ℃ for 24 h and - 10 ℃ for 24 h, respectively, which were 2.23 and 3.13-fold higher than the control levels, respectively. Scanning electron microscopy images indicated that transverse cracks appeared before longitudinal cracks with stronger pretreatment conditions, and holes were found in every sample after this pretreatment. Fourier transform infrared spectroscopy and X-ray diffraction analysis indicated that freeze-thaw pretreatment affected both the crystalline and amorphous regions and disrupted the hydrogen bonds within them. This study provides a physical pretreatment method to improve the efficiency of enzymatic hydrolysis of wheat straw.

Iron-coated biochar alleviates acid accumulation and improves methane production under ammonium enrichment conditions.

The high stress of ammonia-nitrogen in swine manure anaerobic digestion (SMAD) negatively impacts methane yields. Here, the effects of iron-coated biochar in SMAD under different ammonium stresses were investigated. Iron-coated biochar prepared at 500 °C (500BC@Fe) had a large specific surface area (123.2 cm3/g) and an acceptable ammonium adsorption capacity (5.25 mg/g). In SMAD, 500BC@Fe addition effectively broke the thermodynamic barrier from butyrate to acetate and accelerated propionate degradation. It acted as a temporary electron acceptor to promote direct interspecies electron transfer in the initial SMAD stage. As the ammonium stress sharply increased from 400 mg/L to 4000 mg/L, the methanogenesis efficiency decreased from 94.3% to 94.0% and the biochemical methane potential decreased from 189.7 NmL/g VS to 176.1 NmL/g VS. A kinetic analysis showed that the predictive value of SMAD may be calculated more accurately using the Logistic function than the Modified Gompertz equation. This study provides basic theoretical data and important kinetic parameters for the intensive production of iron-coated biochar and its large-scale application in SMAD.

Modified biochar promotes the direct interspecies electron transfer between iron-reducing bacteria and methanogens in high organic loading co-digestion.

High organic loading (HOL) could reduce substrate degradation and methane production. The objective of this study was to investigate the promotion mechanism of iron-modified biochar in HOL co-digestion. The results showed that the specific surface area of iron-modified biochar prepared at 500 °C (500Fe@BC) was 131.7 m2/g. In 12% (w/w) of HOL co-digestion, 500Fe@BC addition enhanced methanogenesis by both aceticlastic and hydrogenotrophic pathways and showed the best methane yield performance. Compared with the non-biochar addition group, an increase of 56.6% and 11% in average methane content and cumulative methane yield was observed in the presence of 500Fe@BC during 25 days of hydraulic retention time. Furthermore, the buffer capacity of HOL co-digestion has been intensified, which attributed to the 500Fe@BC accelerated the hydrolysis of substrates and promoted the consumption of the volatile fatty acids. Moreover, 500Fe@BC promoted the enrichment of iron-reducing bacteria (Clostridium_sensu_stricto_1, Romboutsia) and methanogens (Methanosarcina, Methanobacterium).

Weak-base pretreatment to increase biomethane production from wheat straw.

Weak-base pretreatment of wheat straw was investigated for its ability to improve biomethane production. Anaerobic digestion (AD) was performed on wheat straw pretreated with 3%, 5%, or 7% Na2CO3 as a weak base. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier Transform Infrared Spectroscopy (FTIR) spectra demonstrated disruption of lignocellulosic structures by pretreatment. In the 5% Na2CO3 treatment group, cellulose and hemicellulose were retained effectively, with efficient removal of lignin. The removal rates of cellulose, hemicellulose, and lignin were 27.9%, 20.4%, and 31.0%, respectively, after 5% Na2CO3 pretreatment. The methane content (53.3-77.3%) was improved in the 5% Na2CO3 treatment group, with maximum methane production (307.9 L/kg VS) that was 41.6% higher than that of the untreated sample. Cellulose and hemicelluloses were degraded 59.3% and 56.3% after AD. It took 20 days to reach 80% of the maximum cumulative methane production for the 5% Na2CO3 pretreatment group, which was 4 days faster than the untreated group. These results indicate that 5% Na2CO3 pretreatment improve the lignocellulose structure of wheat straw, allowing better biodegradability of wheat straw in AD for increased biogas production, enhanced methane content, and decreased digestion time.

A technology for strongly improving methane production from rice straw: freeze-thaw pretreatment.

Overcoming the complex three dimensional structure of biomass is a major challenge in enhancing anaerobic digestion (AD) efficacy. Freeze-thaw pretreatment was proposed herein in order to improve methane production from rice straw. The effect was notable: average methane content for group-A (-4 °C) and -B (-20 °C) were A1 (-4 °C, 12 h): 40.0%, A2 (-4 °C, 24 h): 40.5%, A3 (-4 °C, 48 h): 42.2%; B1 (-20 °C, 12 h): 44.2%, B2 (-20 °C, 24 h): 45.7%, B3 (-20 °C, 48 h): 46.0%, the increases were 88.8-99.1% and 108.8-117.2%, respectively, compared with control (CK) (21.2%). Total methane production for group-A and -B were A1: 22.8 mL g-1 TS, A2: 24.7 mL g-1 TS, A3: 27.8 mL g-1 TS; B1: 29.9 mL g-1 TS, B2: 31.3 mL g-1 TS, B3: 32.0 mL g-1 TS, compared with CK (7.6 mL g-1 TS), the increases were 200.0-265.8%, 293.4-321.1%, respectively. The technical digestion time (T 80) was shortened by 8 days. Therefore, the maximum methane production was obtained under conditions of -20 °C and 48 h. This study proposed an efficient pretreatment method that broadens the horizon of improving biomass conversion into bioenergy.

Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation.

In this study, crude cellulase produced by Trichoderma reesei Rut-30 was used to hydrolyze pretreated straw. After the compositions of the hydrolysate of pretreated straw were optimized, the study showed that natural components of pretreated straw without addition of any other components such as (NH4)2SO4, KH2PO4, or Mg(2+) were suitable for citric acid production by Yarrowia lipolytica SWJ-1b, and the optimal ventilatory capacity was 10.0 L/min/L medium. Batch and fed-batch production of citric acid from the hydrolysate of pretreated straw by Yarrowia lipolytica SWJ-1b has been investigated. In the batch cultivation, 25.4 g/L and 26.7 g/L citric acid were yields from glucose and hydrolysate of straw cellulose, respectively, while the cultivation time was 120 hr. In the three-cycle fed-batch cultivation, citric acid (CA) production was increased to 42.4 g/L and the cultivation time was extended to 240 hr. However, iso-citric acid (ICA) yield in fed-batch cultivation (4.0 g/L) was similar to that during the batch cultivation (3.9 g/L), and only 1.6 g/L of reducing sugar was left in the medium at the end of fed-batch cultivation, suggesting that most of the added carbon was used in the cultivation.

Citric acid production in Yarrowia lipolytica SWJ-1b yeast when grown on waste cooking oil.

In this study, citric acid was produced from waste cooking oil by Yarrowia lipolytica SWJ-1b. To get the maximal yield of citric acid, the compositions of the medium for citric acid production were optimized, and our results showed that extra nitrogen and magnesium rather than vitamin B1 and phosphate were needed for CA accumulation when using waste cooking oil. The results also indicated that the optimal initial concentration of the waste cooking oil in the medium for citric acid production was 80.0 g/l, and the ideal inoculation size was 1 × 10(7) cells/l of medium. We also reported that during 10-l fermentation, 31.7 g/l of citric acid, 6.5 g/l of isocitric acid, 5.9 g/l of biomass, and 42.1 g/100.0 g cell dry weight of lipid were attained from 80.0 g/l of waste cooking oil within 336 h. At the end of the fermentation, 94.6 % of the waste cooking oil was utilized by the cells of Y. lipolytica SWJ-1b, and the yield of citric acid was 0.4 g/g waste cooking oil, which suggested that waste cooking oil was a suitable carbon resource for citric acid production.

Direct conversion of pretreated straw cellulose into citric acid by co-cultures of Yarrowia lipolytica SWJ-1b and immobilized Trichoderma reesei mycelium.

The immobilized cellulase-producing mycelium of Trichoderma reesei was found to produce 2.9 U/ml of cellulase activity within 144 h while 2.1 U/ml of cellulase activity was produced within 120 h by the free mycelium of the same strain. When the immobilized mycelium of T. reesei was co-cultivated with the free cells of Yarrowia lipolytica SWJ-1b in flask, Y. lipolytica SWJ-1b could yield 10.7 g/l of citric acid and 3.9 g/l of isocitric acid from 40.0 g/l pretreated straw within 240 h. Under the similar conditions, Y. lipolytica SWJ-1b could yield 32.8 g/l of citric acid and 4.7 g/l of isocitric acid from 40.0 g/l pretreated straw supplemented with 20.0 g/l glucose within 288 h. When the co-cultures were grown in 10-l fermentor, Y. lipolytica SWJ-1b could yield 83.4 g/l of citric acid and 8.7 g/l of isocitric acid from 100.0 g/l of pretreated straw supplemented with 50.0 g/l glucose within 312 h.